Four-channel. fluorescence assay toward six monosaccharides was achieved by employing two novel pyrene-functionalized boronlectins with flexible diboronic acid as receptors. The effects of pH values and aging time on the sensor properties, were thoroughly evaluated by UV-vis, fluorescence spectroscopy and dynamic light scattering. We find. that the fluorescence relative ratios were highly correlated with analyte concentrations at mu M level. The flexibility of the receptors was perceived as an indispensable factor to produce diverse fluorescence signals toward different monosaccharides. Most importantly, integration of four fluorescence channels derived from the two sensors enables an excellent discrimination for all tested monosaccharides at a certain concentration or a concentration range via linear discriminant analysis (LDA). It is proposed that the multiple flexible linkers in the boronlectins could increase their self-adaptive capacity for different analytes, and facilitate the formation of stable boronlectin-sugar aggregate assemblies; In addition, practical sensing,of glucose in the simulative blood and urine was illustrated. to be feasible in the presence of interferences at physiological concentrations.