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题名: Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes
作者: Xu, Shujuan1, 2; Li, Qian1; Xiang, Junfeng1; Yang, Qianfan1; Sun, Hongxia1; Guan, Aijiao1; Wang, Lixia1; Liu, Yan1; Yu, Lijia1; Shi, Yunhua1, 2; Chen, Hongbo1; Tang, Yalin1
刊名: SCIENTIFIC REPORTS
发表日期: 2016-04-21
卷: 6
收录类别: SCI
英文摘要: RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36110
Appears in Collections:分子动态与稳态结构实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Struct Chem Unstable & Stable Speci, Natl Lab Mol Sci, Ctr Mol Sci,Inst Chem, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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