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题名: Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator
作者: Li, Rui1; Zhang, Qing1; Li, Junbai2, 3; Shi, Hualin1
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2016-04-07
卷: 44, 期:6, 页:2554-2563
收录类别: SCI
英文摘要: An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli. The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36108
Appears in Collections:胶体、界面与化学热力学实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
3.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
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