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ICCAS OpenIR  > 分子纳米结构与纳米技术实验室  > 期刊论文
题名: Nitrogen and Sulfur Codoped Reduced Graphene Oxide as a General Platform for Rapid and Sensitive Fluorescent Detection of Biological Species
作者: Chen, Lu1; Song, Liping1; Zhang, Yichi1, 2; Wang, Ping1; Xiao, Zhidong1; Guo, Yuguo2; Cao, Feifei1
关键词: nitrogen and sulfur codoping ; reduced graphene oxide ; quantum dot ; biomolecule sensing ; virus DNA detection
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2016-05-11
卷: 8, 期:18, 页:11255-11261
收录类别: SCI
英文摘要: Nitrogen (N) and sulfur (S) codoped reduced graphene oxide (N,S-rGO) was synthesized through a facile solvothermal process. The introduction of N and S heteroatoms into GO effectively activated the sp(2)-hybridized carbon lattice and made the material an ideal electron/energy acceptor. Such unique properties enable this material to perform, as a general platform for rapid and sensitive detection of various biological species through simple fluorescence quenching and recovering. When quantum dot (QD)-labeled HBV (human being disease-related gene hepatitis B virus DNA) and HIV (human being disease-related gene human immunodeficiency virus DNA) molecular beacon probes were mixed with N,S-rGO, QD fluorescence was, quenched; when target HBV and HIV DNA were added, QD fluorescence was recovered. By the recovered fluorescence intensity, the target virus DNA detection limits were reduced to 2.4 nM for HBV and 3.0 nM for HIV with detection time of less than 5 min. It must be stressed out that different viruses in the same homogeneous aqueous media could be discriminated and quantified simultaneously through choosing diverse QD probes with different colors. Moreover, even one mismatched target DNA could be distinguished using this method. When altering the molecular beacon loop domain to protein aptamers, this sensing strategy was also able to detect thrombin and IgE in 5 min with detection limits of 0.17 ng mL(-1) and 0.19 ng mL(-1), respectively, which was-far more rapid and sensitive than bare GO-based fluorescence detection strategy.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36077
Appears in Collections:分子纳米结构与纳米技术实验室_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
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