中国科学院化学研究所机构知识库
Advanced  
ICCAS OpenIR  > 分析测试中心  > 期刊论文
题名: Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain
作者: Zhang, Yangyang1; Wang, Jun2, 3; Liu, Jian'an1; Han, Juanjuan1; Xiong, Shaoxiang1; Yong, Weidong2, 3; Zhao, Zhenwen1, 4
刊名: SCIENTIFIC REPORTS
发表日期: 2016-05-04
卷: 6
收录类别: SCI
英文摘要: Gangliosides are a family of complex lipids that are abundant in the brain. There is no doubt the investigations about the distribution of gangliosides in brian and the relationship between gangliosides and Alzheimer's disease is profound. However, these investigations are full of challenges due to the structural complexity of gangliosides. In this work, the method for efficient extraction and enrichment of gangliosides from brain was established. Moreover, the distribution of gangliosides in brain was obtained by matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). It was found that 3-aminoquinoline (3-AQ) as matrix was well-suited for MALDI MS analysis of gangliosides in negative ion mode. In addition, the pretreatment by ethanol (EtOH) cleaning brain section and the addition of ammonium formate greatly improved the MS signal of gangliosides in the brain section when MALDI MSI analysis was employed. The distribution of ganliosides in cerebral cortex, hippocampus and cerebellum was respectively acquired by electrospray ionization (ESI) MS and MALDI MSI, and the data were compared for reliability evaluation of MALDI MSI. Further, applying MALDI MSI technology, the distribution of gangliosides in amyloid precursor protein transgenic mouse brain was obtained, which may provide a new insight for bioresearch of Alzheimer's disease (AD).
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36042
Appears in Collections:分析测试中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem,Beijing Mass Spectrum Ctr, Beijing, Peoples R China
2.Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing 100730, Peoples R China
3.Peking Union Med Coll, Beijing 100021, Peoples R China
4.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yangyang]'s Articles
[Wang, Jun]'s Articles
[Liu, Jian'an]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yangyang]‘s Articles
[Wang, Jun]‘s Articles
[Liu, Jian'an]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院化学研究所 - Feedback
Powered by CSpace