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题名: Super-resolution imaging and tracking of TGF-beta receptor II on living cells
作者: Ye, Zi1; Li, Nan1; Zhao, Libo1; Sun, Yahong1; Ruan, Hefei1; Zhang, Mingliang1; Yuan, Jinghe1; Fang, Xiaohong1
关键词: Single-particle tracking ; Photoactivated localization microscopy ; Single-molecule fluorescence imaging ; TGF-beta receptor II ; Membrane diffusion
刊名: SCIENCE BULLETIN
发表日期: 2016-04-01
卷: 61, 期:8, 页:632-638
收录类别: SCI
英文摘要: Single-particle tracking photoactivated localization microscopy (sptPALM) has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-beta type II receptor (T beta RII) at high expression level using both traditional single-particle tracking (SPT) and sptPALM. The ligand-induced aggregation of TbRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36033
Appears in Collections:分子纳米结构与纳米技术实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
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