中国科学院化学研究所机构知识库
Advanced  
ICCAS OpenIR  > 活体分析化学实验室  > 期刊论文
题名: A Bis(methylpiperazinylstyryl)phenanthroline as a Fluorescent Ligand for G-Quadruplexes
作者: Wu, Shangrong1, 2; Wang, Linlin1; Zhang, Nan1; Liu, Ying1; Zheng, Wei1; Chang, Ang1, 2; Wang, Fuyi1; Li, Songqin2; Shangguan, Dihua1
关键词: cytotoxicity ; fluorescent probes ; G-quadruplexes ; ligands ; phenanthroline derivative
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2016-04-18
卷: 22, 期:17, 页:6037-6047
收录类别: SCI
英文摘要: G-quadruplex (G4)-forming sequences are prevalent in the genome and are considered to play important roles in gene regulation, and hence have been viewed as potential therapeutic targets in oncology. However, the structures and functions of most G4s in the genome are poorly understood. Therefore, the development of fluorescent probes and ligands for G4s is important for G4 research and drug discovery. Herein, we report a new G4 ligand, 2,9-bis[4-(4-methylpiperazin-1-yl)styryl]-1,10-phenanthroline (BMSP), which was synthesized by a simple process. BMSP exhibits almost no fluorescence in aqueous buffer. The interaction of BMSP with G4s greatly enhances its fluorescence with a large Stokes' shift of 160nm. Antiparallel human telomeric G4s exhibit the strongest binding affinity (K-d approximate to 0.13m) to BMSP and induce a fluorescence enhancement of up to 150-fold. BMSP binds to G4s through - stacking on the terminal G-quartets. BMSP can enter live cells, and it strongly inhibits the growth of cancer cells rather than causing cell death. Our results suggest that BMSP has the potential to serve both as a fluorescent probe for some G4s and as a chemotherapeutic agent for cancer treatment.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/36012
Appears in Collections:活体分析化学实验室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Xiangtan Univ, Coll Chem, Xiangtan 411100, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, Shangrong]'s Articles
[Wang, Linlin]'s Articles
[Zhang, Nan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, Shangrong]‘s Articles
[Wang, Linlin]‘s Articles
[Zhang, Nan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院化学研究所 - Feedback
Powered by CSpace