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题名: Online electrochemical system as an in vivo method to study dynamic changes of ascorbate in rat brain during 3-methylindole-induced olfactory dysfunction
作者: Li, Lijuan1; Zhang, Yinghong1; Hao, Jie2; Liu, Junxiu1; Yu, Ping2; Ma, Furong1; Mao, Lanqun2
刊名: ANALYST
发表日期: 2016
卷: 141, 期:7, 页:2199-2207
收录类别: SCI
英文摘要: This study demonstrates the application of an online electrochemical system (OECS) as an in vivo method to investigate the dynamic change of microdialysate ascorbate in the olfactory bulb (OB) of rats during the acute period of olfactory dysfunction induced by intraperitoneal (i.p.) injection of 3-methylindole (3-MI). The OECS is developed by directly coupling an electrochemical detector to in vivo microdialysis for the direct monitoring of ascorbate. The system benefits from the good electrochemical activity of single-walled carbon nanotubes towards the oxidation of ascorbate and exhibits high selectivity, good stability, reproducibility and linearity for the measurement of ascorbate in the OB under physiological conditions. With this method, the basal level of microdialysate ascorbate in the OB is determined to be 48.64 +/- 5.44 mu M. The administration of 3-MI clearly increases the microdialysate ascorbate in the OB after 3-MI treatments and this increase is obviously alleviated by intravenous administration of ascorbate and glutathione (GSH) within 10 min after i.p. injection of 3-MI. These observations with the OECS suggest that ascorbate may be involved in chemical processes during the early stages of 3-MI-induced olfactory dysfunction. This study essentially validates the OECS as an in vivo method for effective measurement of ascorbate in the OB in rat brain and such a method will find interesting applications in investigating chemical process associated with ascorbate underlying olfactory dysfunction.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35932
Appears in Collections:活体分析化学实验室_期刊论文

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作者单位: 1.Peking Univ, Dept Otolaryngol Head & Neck Surg, Hosp 3, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
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