A novel photoelectrochemical (PEC) analyzing platform was fabricated based on tungsten-doped Bi2S3 (W-Bi2S3) composite and electropolymerized poly(thiophenyl-3-boronic acid) (PTBA) for detecting subgroup J of avian leukosis virus (ALVs-J). W-Bi2S3 composite was synthesized using a facile hydrothermal method without any organic reagents. Benefiting from the appropriate energy level cascade between PTBA and W-Bi2S3, the PTBA/W-Bi2S3/ITO electrode exhibited improved PEC performance. Based on the reaction between boric acid and sialic acid on the surface of target virus, boronic acids were used as probing receptors for capturing ALVs-J. Alkaline phosphatase assembled on the modified electrode surface was used to catalyze the hydrolysis of ascorbic acid 2-phosphate in situ producing ascorbic acid for electron donating. The proposed PEC sensor for ALVs-J assay showed a wide linear range, high sensitivity and lower detection limit. This method provides a rapid, effective and reusable platform for screening the ALVs-J-infected poultry. (C) 2016 Published by Elsevier B.V.