中国科学院化学研究所机构知识库
Advanced  
ICCAS OpenIR  > 活体分析化学实验室  > 期刊论文
题名: Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging
作者: Wang, Xiao1, 2; Xu, Jiying1; Liu, Chanjuan1, 2; Chen, Yi1, 2, 3
刊名: RSC ADVANCES
发表日期: 2016
卷: 6, 期:26, 页:21900-21906
收录类别: SCI
英文摘要: The DNA-targeting platinum complex cisplatin is one of the most successful drugs for clinical treatment of solid tumors, and several new analogues of transplatin have shown cytostatic activity recently. The specific recognition of platinated DNA with cellular proteins is of great interest for better understanding of the pharmaceutical mechanisms. Herein, a surface plasmon resonance imaging (SPRi) method to differentiate the interaction between the protein human high mobility group box 1 (HMGB1) and DNAs, and human nuclear protein positive cofactor 4 (PC4) and DNAs has been developed. Four kinds of DNAs were immobilized on the gold films including platinated-DNA adducts (cisplatin and trans[PtCl2(NH3)(thiazole)] (trans-PtTz) damaged DNAs; referred to as cisPt-DNA1 and transPtTz-DNA2, respectively) and native DNAs (DNA1 and DNA2, as controls). The validation of the method has been proven by the specific recognition of HMGB1 and cisPt-DNA1 first. The results obtained indicated that the PC4 was more likely to bind to platinated DNAs (cisPt-DNA1 and transPtTz-DNA2) than the native DNAs. Temperature-dependent kinetics and thermodynamics revealed that the recognition behavior was not affected by a temperature changefrom 15 degrees C to 42 degrees C. This label-free method provides authentic results, as the controls can be simultaneously determined by a single chip under the same conditions, and this makes it suited for other high throughput analyses of interactions between drug-damaged DNAs and proteins to better understand the activity/inactivity mechanisms of drugs and drug screening/discovery.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35899
Appears in Collections:活体分析化学实验室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Xiao]'s Articles
[Xu, Jiying]'s Articles
[Liu, Chanjuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Xiao]‘s Articles
[Xu, Jiying]‘s Articles
[Liu, Chanjuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院化学研究所 - Feedback
Powered by CSpace