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ICCAS OpenIR  > 分子纳米结构与纳米技术实验室  > 期刊论文
题名: Computational design of peptide-Au cluster probe for sensitive detection of alpha(IIb)beta(3) integrin
作者: Zhao, Lina1; Zhai, Jiao1; Zhang, Xuejie1, 2; Gao, Xueyun1; Fang, Xiaohong2; Li, Jingyuan1
刊名: NANOSCALE
发表日期: 2016
卷: 8, 期:7, 页:4203-4208
收录类别: SCI
英文摘要: We have designed a novel peptide-Au cluster probe to specifically bind to alpha(IIb)beta(3) integrin. As indicated by molecular dynamics (MD) simulations, the binding mode of the native ligand of alpha(IIb)beta(3) integrin, gamma C peptide, can be realized by the designed probe. More importantly, the peptide-Au probe can provide multiple coating peptides to form additional salt bridges with protein, and the binding stability of the probe is comparable to the native ligand. The designed probe was then successfully synthesized. The specific binding in a cellular environment was validated by colocalization analysis of confocal microscopy. In addition, the binding affinity was confirmed by atomic force microscopy (AFM) based single molecule force spectroscopy. Our results suggest the combination of computational design and experimental verification can be a useful strategy for the development of nanoprobes.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35858
Appears in Collections:分子纳米结构与纳米技术实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100049, Peoples R China
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