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题名: A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition
作者: Tang, Cong1; Qian, Zhaosheng1; Huang, Yuanyuan1; Xu, Jiamin1; Ao, Hang1; Zhao, Meizhi1; Zhou, Jin2; Chen, Jianrong1; Feng, Hui1
关键词: Carbon quantum dots ; Alkaline phosphatase (ALP) activity ; Real-time fluorometric assay ; Photoinduced electron transfer (PET)
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2016-09-15
卷: 83, 页:274-280
收录类别: SCI
英文摘要: A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on beta-cyclodextrin-modified carbon quantum dots (beta-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-amino phenyl boronic acid to produce boronic acid-functionalized CQDs, and then further modified with hydropropyl beta-cyclodextrins (beta-CD) through B-O bonds to form beta-CD-CQDs nanoprobe. p-Nitrophenol phosphate disodium salt is used as the substrate of ALP, and can hydrolyze to p-nitrophenol under the catalysis of ALP. The resulting p-nitrophenol can enter the cavity of beta-CD moiety in the nanoprobe due to their specific host-guest recognition, where photoinduced electron transfer process between p-nitrophenol and CQDs takes place to efficiently quench the fluorescence of the probe. The correlation between quenched fluorescence and ALP level can be used to establish quantitative evaluation of ALP activity in a broad range from 3.4 to 100.0 U/L with the detection limit of 0.9 U/L. This assay shows a high sensitivity to ALP even in the presence of a very high concentration of glucose. This study demonstrates a good electron donor/acceptor pair, which can be used to design general detection strategy through PET process, and also broadens the application of host-guest recognition for enzymes detection in clinical practice. (C) 2016 Elsevier B.V. All rights reserved.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35761
Appears in Collections:活体分析化学实验室_期刊论文

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作者单位: 1.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
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