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ICCAS OpenIR  > 分子纳米结构与纳米技术实验室  > 期刊论文
题名: Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging
作者: Zhou Qian1, 2, 3; Yu Jian-Qiang4; Zhao Li-Bo3; Li De-Sheng5; Wu Kui6; Zhu Jian-Hua1, 2; Yuan Jing-He3; Fang Xiao-Hong3
关键词: Super-resolution microscopy ; Subtraction imaging ; Non-fluorescence imaging ; Optical scattering imaging
刊名: ACTA PHYSICO-CHIMICA SINICA
发表日期: 2016-05-15
卷: 32, 期:5, 页:1123-1128
收录类别: SCI
英文摘要: The existing form of super-resolution microscopy based on specific fluorescent tagging is unable to obtain super-resolution images of non-fluorescent samples. Hence, we have developed optical subtraction microscopy for obtaining super-resolution imaging in such cases. This method is based on image subtraction between the two optical scattering images from general confocal excitation and doughnut-shaped excitation, respectively. Unlike super-resolution fluorescence microscopy, subtraction microscopy requires no preprocessing of the sample, and the excitation power can be kept low to avoid sample damage. The non-fluorescent imaging of gold nanobeads and polymer nanofibers has been realized to demonstrate the feasibility of super-resolution subtraction microscopy. The lateral resolution decreases to 215 nm (0.33 lambda, 1 lambda = 650 nm) in subtraction imaging, and greater imaging detail of the sample is achieved via optical scattering.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35760
Appears in Collections:分子纳米结构与纳米技术实验室_期刊论文

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作者单位: 1.Sichuan Univ, Dept Phys, Chengdu 610064, Peoples R China
2.Minist Educ, Key Lab High Energy Dens Phys & Technol, Chengdu 610064, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
4.North China Univ Technol, Coll Sci, Beijing 100144, Peoples R China
5.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China
6.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
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