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题名: A Cyanine Dye to Probe Mitophagy: Simultaneous Detection of Mitochondria and Autolysosomes in Live Cells
作者: Liu, Ying1, 2; Zhou, Jin1, 6; Wang, Linlin1, 2; Hu, Xiaoxiao3, 4; Liu, Xiangjun1; Liu, Meirong1; Cao, Zehui1; Shangguan, Dihua1, 2; Tan, Weihong3, 4, 5
刊名: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
发表日期: 2016-09-28
卷: 138, 期:38, 页:12368-12374
收录类别: SCI
英文摘要: Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents in a lysosome-dependent manner. To probe this process, two different fluorescent dyes specific for mitochondria and lysosomes, respectively, are often used in combination. However, current fluorescent dyes for lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes. Therefore, we herein report a cyanine dye, HQO, which can simultaneously probe mitochondria and autolysosomes in live cells by exhibiting different fluorescence properties. HQO selectively accumulates in mitochondria but then transforms to the protonated HQOH(+) form with the decrease of pH when dysfunctional mitochondria evolve into autolysosomes. Since HQO and HQOH(+) exhibit different absorption and emission with Ex/Em at 530/650 and 710/750 nm, respectively, in a low polarity environment, such as that found in micelles, they are uniquely suited to monitor mitophagy with the ability to distinguish autolysosomes from other lysosomes.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35653
Appears in Collections:活体分析化学实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China
4.Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
5.Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Hlth Canc Ctr,UF Genet Inst,McKnight Brain Inst, Gainesville, FL 32611 USA
6.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
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