中国科学院化学研究所机构知识库
Advanced  
ICCAS OpenIR  > 活体分析化学实验室  > 期刊论文
题名: An Online Electrochemical System for Continuously Monitoring Uric Acid Change following Rabbit Kidney following Ischemia-reperfusion Injury
作者: Lin, Yuqing1, 2; Li, Bo2; Hao, Jie1; Xiao, Tongfang1; Yang, Yan1; Yu, Ping1; Mao, Lanqun1
关键词: Online Electrochemical System ; Uric Acid ; Microfluidic Chip ; Kidney Ischemia-reperfusion
刊名: ELECTROCHIMICA ACTA
发表日期: 2016-08-10
卷: 209, 页:132-137
收录类别: SCI
英文摘要: In vivo monitoring of uric acid (UA) with high reliability and robustness is of great physiological and pathological importance because UA, as one kind of the most important chemical species in the biological systems, plays a great role in some physiological processes and is used as the biomarkers for early diagnosis of several diseases. In this study, a microfluidic chip-based online electrochemical system (OECS) is developed for the first time for continuously monitoring UA in rabbit kidney. To establish the OECS for UA monitoring, a single-channel microfluidic chip is developed into an electrochemical cell by incorporating a single-walled carbon nanotubes (SWNTs)-modified indium-tin oxide (ITO) electrode as working electrode, an Ag/AgCl wire as reference electrode, and a Pt tube as counter electrode. To completely remove ascorbic acid and thus eliminate its interference toward UA detection in such a single-channel microfluidic chip, ascorbate oxidase (AOx) is crosslinked onto both the etched ITO glass in the upstream channel and the SWNT-modified ITO electrode in the downstream. With the as-prepared microfluidic chip-based electrochemical cell as the detector for selective detection of UA, an online electrochemical system is successfully established by directly coupling the microfluidic chip-based electrochemical detector with in vivo microdialysis. The system exhibits a good linear response, high stability and good in vivo selectivity and is thus able to continuously sense UA change in rabbit kidney following ischemia-reperfusion injury. The study essentially offers a new OECS for in vivo continuous monitoring of UA. (C) 2016 Elsevier Ltd. All rights reserved.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35594
Appears in Collections:活体分析化学实验室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Lin, Yuqing]'s Articles
[Li, Bo]'s Articles
[Hao, Jie]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Lin, Yuqing]‘s Articles
[Li, Bo]‘s Articles
[Hao, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院化学研究所 - Feedback
Powered by CSpace