ICCAS OpenIR  > 活体分析化学实验室
Self-Assembled Nanostructures Based on Activatable Red Fluorescent Dye for Site-Specific Protein Probing and Conformational Transition Detection
Yu, Yang1,2; Huang, Yanyan1,2; Hu, Fang1,2; Jin, Yulong1,2; Zhang, Guanxin1,2; Zhang, Deqing1,2; Zhao, Rui1,2
2016-06-21
Source PublicationANALYTICAL CHEMISTRY
Volume88Issue:12Pages:6374-6381
AbstractSmart and versatile nanostructures have demonstrated their effectiveness for biomolecule analysis and show great potential in digging insights into the structural/functional relationships. Herein, a nanoscale molecular self-assembly was constructed for probing the site-specific recognition and conformational changes of human serum albumin (HSA) with tunable size and emission. A tetraphenylethylene derivative TPE-red-COOH was used as the building block for tailoring fluorescence-silent nanoparticles. The highly specific and sensitive response to HSA was witnessed by the fast turn-on of the red fluorescence and simultaneous disassembly of the nanostructures, whereas various endogenous biomolecules cannot induce such response. The mechanism investigation indicates that the combination of multiple noncovalent interactions is the driving force for disassembling and trapping TPE-red-COOH into HSA. The resultant restriction of intramolecular rotation of TPE-red-COOH in the hydrophobic cavity of HSA induces the significant red emission. By using the fluorescence activatable nanosensor as the structural indicator, the stepwise conformational transitions of HSA during denaturing and the partial refolding of subdomain IIA of HSA were facilely visualized. Benefiting from its activatable signaling, sensitivity, and simplicity, such molecular assembly provides a kind of soft nanomaterial for site-specific biomolecule probing and conformational transition detection concerning their structure, function, and biomedical characteristics.
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/35543
Collection活体分析化学实验室
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst & Organ So, Inst Chem, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yu, Yang,Huang, Yanyan,Hu, Fang,et al. Self-Assembled Nanostructures Based on Activatable Red Fluorescent Dye for Site-Specific Protein Probing and Conformational Transition Detection[J]. ANALYTICAL CHEMISTRY,2016,88(12):6374-6381.
APA Yu, Yang.,Huang, Yanyan.,Hu, Fang.,Jin, Yulong.,Zhang, Guanxin.,...&Zhao, Rui.(2016).Self-Assembled Nanostructures Based on Activatable Red Fluorescent Dye for Site-Specific Protein Probing and Conformational Transition Detection.ANALYTICAL CHEMISTRY,88(12),6374-6381.
MLA Yu, Yang,et al."Self-Assembled Nanostructures Based on Activatable Red Fluorescent Dye for Site-Specific Protein Probing and Conformational Transition Detection".ANALYTICAL CHEMISTRY 88.12(2016):6374-6381.
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