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题名: Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique
作者: Jiang, Chao1, 2; Yuan, Yuan1; Yang, Guang1; Jin, Yan1; Liu, Libing3; Zhao, Yuyang1; Huang, Luqi1
刊名: SCIENTIFIC REPORTS
发表日期: 2016-10-21
卷: 6
收录类别: SCI
英文摘要: Inaccurate labeling of materials used in herbal products may compromise the therapeutic efficacy and may pose a threat to medicinal safety. In this paper, a rapid (within 3 h), sensitive and visual colorimetric method for identifying substitutions in terminal market products was developed using cationic conjugated polymer-based fluorescence resonance energy transfer (CCP-based FRET). Chinese medicinal materials with similar morphology and chemical composition were clearly distinguished by the single-nucleotide polymorphism (SNP) genotyping method. Assays using CCP-based FRET technology showed a high frequency of adulterants in Lu-Rong (52.83%) and Chuan-Bei-Mu (67.8%) decoction pieces, and patented Chinese drugs (71.4%, 5/7) containing Chuan-Bei-Mu ingredients were detected in the terminal herbal market. In comparison with DNA sequencing, this protocol simplifies procedures by eliminating the cumbersome workups and sophisticated instruments, and only a trace amount of DNA is required. The CCP-based method is particularly attractive because it can detect adulterants in admixture samples with high sensitivity. Therefore, the CCP-based detection system shows great potential for routine terminal market checks and drug safety controls.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35468
Appears in Collections:有机固体实验室_期刊论文

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作者单位: 1.China Acad Chinese Med Sci, State Key Lab Breeding Base Daodi Herbs, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
2.Beijing Normal Univ, Coll Resources, Beijing Area Major Lab Protect & Utilizat Tradit, Beijing 100875, Peoples R China
3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China
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