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题名: A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein
作者: Lin, Dayong1; Fei, Xuening1, 2; Li, Ran2; Gu, Yingchun2, 3; Tang, Yalin3; Zhou, Jianguo2; Zhang, Baolian2
关键词: Spectra properties ; Fluorescent probe ; Protein ; Specific
刊名: JOURNAL OF FLUORESCENCE
发表日期: 2016-07-01
卷: 26, 期:4, 页:1511-1520
收录类别: SCI
英文摘要: A novel fluorescent dye, 1-(2-hydroxyethyl)-4-((E)-2-(3-benzothiazol-2yl-9-ethyl-carbazole-3yl)vinyl) pyridinium bromide, was synthesized for determination of protein and its structure was characterized by H-1 NMR. Photophysics of the new probe in different solvents has been delineated in this paper, the new fluorescent molecular dye exhibited a large stokes-shifted and fluorescence quantum yields in organic solvent. The photostability and thermostability of the new dye were also studied and the results suggested the stable was excellent. The interactions of the dye with bovine serum albumin (BSA) , Human serumal bumin (HSA) and calf thymus deoxyribonucleic acid (ctDNA) were studied by fluorescence and absorption spectroscopy. The binding constant for BSA, HSA and DNA were calculated to be 8.91 x 10(7), 1.86 x 10(6) and 2.9 x 10(4), respectively. The experimental results indicated a potential value of the new dye for biomarker.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35370
Appears in Collections:分子动态与稳态结构实验室_期刊论文

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作者单位: 1.Tianjin Univ, Sch Envirnmental Sci & Engn, 92 Weijin Rd, Tianjin 300072, Peoples R China
2.Tianjin Chengjian Univ, Sch Sci, 26 Jinjing Rd, Tianjin 300384, Peoples R China
3.Chinese Acad Sci ICCAS, Inst Chem, Zhongguancun North First St 2, Beijing 100190, Peoples R China
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