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ICCAS OpenIR  > 分子纳米结构与纳米技术实验室  > 期刊论文
题名: Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients
作者: Liu, Shijie1, 2, 3; Tian, Zuhong2, 3; Zhang, Lei4; Hou, Shuang5; Hu, Sijun2, 3; Wu, Junshen1; Jing, Yuming1; Sun, Huimin1; Yu, Fei1; Zhao, Libo6; Wang, Ruoxiang7; Tseng, Hsian-Rong5; Zhau, Haiyen E.7; Chung, Leland W. K.7; Wu, Kaichun2, 3; Wang, Hao8; Wu, Jason Boyang9; Nie, Yongzhan2, 3; Shao, Chen1, 2, 3
关键词: carbonic anhydrase 9 ; CD147 ; circulating tumor cells ; renal cell carcinoma
刊名: ONCOTARGET
发表日期: 2016-09-13
卷: 7, 期:37, 页:59877-59891
收录类别: SCI
英文摘要: Circulating tumor cells (CTCs) have emerged as promising tools for noninvasive cancer detection and prognosis. Most conventional approaches for capturing CTCs use an EpCAM-based enrichment strategy, which does not work well in cancers that show low or no expression of EpCAM, such as renal cell carcinoma (RCC). In this study, we developed a new set of cell surface markers including CA9 and CD147 as alternative CTC-capture antigens specifically designed for RCC patients. We showed that the expression of both CA9 and CD147 was prevalent in a RCC patient cohort (n= 70) by immunohistochemical analysis, with both molecules in combination covering 97.1% of cases. The NanoVelcro platform combined with CA9-/CD147-capture antibodies demonstrated significantly higher efficiency for capturing both CTC-mimicking renal cancer cells and RCC CTCs in peripheral blood, compared to the conventional EpCAM-based method. Using immunofluorescence cytological validation at the single-cell level, we were able to identify bona fide CTCs in RCC patient blood following the well-accepted criteria in our CTC-capture system. We further demonstrated a significant association of CTC numbers as well as the CTC expression status of Vimentin, a mesenchymal marker, with disease progression, including pathologic features and clinical staging. These results provide new insights into developing novel, effective targets/approaches for capturing CTCs, making CTCs a valuable tool for improved cancer detection, prognosis and treatment in RCC.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.iccas.ac.cn/handle/121111/35097
Appears in Collections:分子纳米结构与纳米技术实验室_期刊论文

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作者单位: 1.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710032, Shaanxi, Peoples R China
2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi, Peoples R China
4.Fourth Mil Med Univ, Dept Epidemiol, Xian 710032, Shaanxi, Peoples R China
5.Univ Calif Los Angeles, Calif Nanosyst Inst, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA
6.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
7.Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Dept Med, Urooncol Res Program, Los Angeles, CA 90048 USA
8.Natl Ctr Nanosci & Nanotechnol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
9.Washington State Univ, Coll Pharm, Dept Pharmaceut Sci, Spokane, WA 99202 USA
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